Product Data Sheet

Recovery

1.       Obtain an LB agar plate with the appropriate antibiotic.

2.       Using a sterile pipette tip, touch the bacteria growing within the punctured area of the stab culture. (A sterilized wire loop or sterile toothpick can be used in place of a sterile pipette tip.)

3.       Run this tip lightly over a section of the plate, as shown in the figure, to create streak #1.

4.       Using another sterile pipette tip, pass through streak #1 and spread the bacteria over a second section of the plate, to create streak #2.

5.       Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to create streak #3.

6.       Grow overnight in a 37 ^o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7.       In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate to obtain single colonies.

载体信息：

Sequence:

1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga

       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg

      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg

      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca

      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc

      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc

      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc

      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa

      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag

      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta

      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc

      661 atgggcccta aaaagaagcg taaagtcgcc atcgatcagc tcaccatggt gtttccttct

      721 gggcagatct caaaccaggc cctggcctta gcaccgtcct ctgccccagt ccttgcccag

      781 accatggtcc cttcctcagc catggtacct ctggctcagc ccccagctcc tgccccagtt

      841 ctaaccccgg gtcctcccca gtccctgtct gcacctgttc caaagagcac ccaggctggg

      901 gaaggcacgc tgtcggaagc cctgctgcac ctgcagtttg atgctgatga agacttgggg

      961 gccttgcttg gcaacagcac agacccagga gtgttcacag acctggcatc tgtggacaac

     1021 tcagagtttc agcagctcct gaaccagggt gtgtccatgt ctcactccac agctgagccc

     1081 atgctgatgg agtaccctga agctataact cgcctggtga cagggtccca gaggccccct

     1141 gacccagctc ccacacccct ggggacctcg gggcttccca atggtctctc cggagatgaa

     1201 gacttctcct ccattgcgga catggacttc tctgctctgc tgagtcagat cagctccagc

     1261 ggccaaggat ccagcccggg cgaattcgga agcttgcggc cgcgtcgacc tctagactgc

     1321 agctcgaggg ggggcccggt accaggtaag tgtacccaat tcgccctata gtgagtcgta

     1381 ttacaattca ctcgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggagatc

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