
pBApo-CMV-pur载体质粒
产品名称: pBApo-CMV-pur载体质粒
英文名称: pBApo-CMV-pur载体质粒
产品编号: BiovectorPBAPOCP
产品价格: 0
产品产地: Biovector Inc. USA
品牌商标: Biovector, Addgene, ATCC, Invi
更新时间: null
使用范围: null
- 联系人 :
- 地址 : 北京市西直门外大街19号BioVector NTCC典型培养物保藏中心
- 邮编 : 100080
- 所在区域 : 北京
- 电话 : 189****8599 点击查看
- 传真 : 点击查看
- 邮箱 : Biovector@163.com
Product Data Sheet
Order ID |
Name |
Description |
BiovectorPBAPOCP |
pBApo-CMV-pur |
pBApo-CMV-pur 15uL DNA. AmpR.Storage:-20℃ |
Standard heat-shock transformation of chemically competent bacteria
- Take competent cells out of -80°C and thaw on ice.
- Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator.
- Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times.
- Place the competent cell/DNA mixture on ice for 20-30min.
- Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min.
- Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min.
- Plate all of the transformation onto a 10cm LB agar plate containing the appropriate antibiotic.
- Incubate plates at 37°C overnight.
载体信息:
载体名称: |
pBApo-CMV-Pur |
质粒类型: |
哺乳动物细胞表达载体 |
高拷贝/低拷贝: |
低拷贝 |
克隆方法: |
限制性内切酶,多克隆位点 |
启动子: |
CMV |
载体大小: |
4358 bp |
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3 测序引物及序列: |
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载体标签: |
无标签 |
载体抗性: |
氨苄青霉素 |
筛选标记: |
嘌呤霉素(Puromycin) |
克隆菌株: |
TOP10, DH5α, JM109 |
宿主细胞(系): |
常规细胞系,293、CV-1、CHO等 |
备注: |
pBApo-CMV-Pur载体可用来表达microRNA和其它转录产物; |
产品目录号: |
3241 |
稳定性: |
瞬表达 或 稳表达 |
组成型/诱导型: |
TUNEology 波长可调检测卡盒--> |