Product Information
 
Product   Name
Catalog No.
Size
Price (￥)
Pyrophosphatase, Inorganic   GMP-grade (1 U/μL)
10620ES10
10 U
645
10620ES60
100 U
4855
10620ES80
1,000 U
Inquiry
10620ES99
40 KU
Inquiry
 
Product Description
 
This product is an inorganic pyrophosphatase derived from recombinant expression in E. coli. It is an enzyme that can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. This is a high-energy reaction, so this reaction can be coupled to some thermodynamically unfavorable transformations in order to drive these transformations to completion. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate. In molecular biology, it can be used to increase RNA yield in reverse transcription reaction.
This product is produced in accordance with GMP process requirements, and the product is provided in liquid form
 
Product Properties
 
Source
Recombinant E.coli with   Pyrophosphatase gene from yeast
Optimum   Temperature
25℃
Storage   Buffer
50 mM   Tris-HCl pH 7.9, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% （v/v）TritonX-100, 50%（v/v）glycerin
Unit   Definition
One unit (U) is the amount of enzyme needed   to catalyze the hydrolysis of PPI per minute to produce 1 μmol Pi.（The standard reaction is: 500 μL system contains 100 mM   Tris-HCl pH7.2, 2 mM MgCl2, 2 mM Ppi; reaction at 25℃ for 10 min）
 
Contents
 
Catalog No.
Name
Catalog No./Specification
 
10620ES10
(10 U)
10620ES60
(100 U)
10620ES80
(1,000   U)
10620ES99
(40 KU)
10620
Pyrophosphatase, Inorganic   GMP-grade (1 U/μL)
10 μL
100 μL
1 mL
40 mL
 
Shipping and Storage
 
The Pyrophosphatase, Inorganic GMP-grade products shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.
 
Notes
 
1. This product is active in a variety of reaction buffers. Usually, this product can be directly connected in experiments such as IVT.
2.The optimal reaction temperature of this product is 25℃, it is active at 16-37℃, 65℃ can inactivate the enzyme for 10 minutes.
3. For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this
 
HB220701